Introduction
- Ozone therapy is a branch of medicine that uses ozone to cure or manage various disease.
- 0.5% to 5% ozone and 95-99.5% oxygen make up medical ozone.
- 3 molecule of oxygen combine to form the colorless gas of ozone (o3).
- Oxygen is an important factor to sustain life. A human being can live upto 3 minutes without oxygen. Lack of oxygen is one of the most common causative factor known to promote many diseases such as diabetes, cancer etc.
- The elementary form of O2 is used for biological oxidation process and is centralized to cells in obtaining their energy (ATP), thus aiding in the essential of organs. Ozone gas is a powerful oxygen molecule containing 3 atoms of oxygen.
- According to Dorland’s medical dictionary: ozone is a bluish explosive gas, which is an allotropic and more active form of oxygen.
History
- Christian Friedrich Schonbein (1799–1868) discovered ozone in 1840. Can also smell it during a thunderstorm.
- The concept of ozone generation was first practically applied by- chemist Werner von Simens.
- The first medical application seems to have been the use of ozone for treating post-traumatic gangrene in German soldiers during the 1st world war.
- However, a big step forward was the invention of a reliable ozoniser for medical use by the physicist Joachim Hansler (1908–1981).
- The idea to use ozone in medicine developed slowly during the last century and it was stimulated by the lack of antibiotics and the disinfectant properties of ozone. Not surprisingly a swiss dentist, E.A. Fisch (1899-1966) was the first to use ozone in his practice.
- Dr. E. Payr (1871–1946), a surgeon had to be treated for a gangrenous pulpite and soon realized the efficacy of the ozone treatment in surgery.
- In 1936, in France, Dr. P. Aubourg proposed to use the insufflation of oxygen-ozone into the rectum to treat chronic colitis and fistulae.
Medical Ozone Generator
Medical ozone generator is a device which generates ozone molecules for various clinical applications by using pure medical grade oxygen.
It is generated by using 3 techniques
- Corona discharge
- UV rays – ambient air
- Cold plasma
Parameters and units used
C= Concentration
V= Volume
F= Flow rate in ml/min
T= Time
D= Dosage in mcg
Ozone toxicity

Mechanism of action
- The administration of ozone causes a number of biochemical, pharmacological and psycho-neuro-immunological reactions to take place in a patient who is an essential part of the process.
- By causing the release of natural antioxidants including superoxide dismutase, glutathione peroxidase, glutathione s- transferase and catalase, ozone helps to reduce inflammation.
- Ozone has antimicrobial properties.
- Modulates immunity.
- O3therapy induces moderate oxidative stress when interacting with lipids. This interaction increases endogenous production of antioxidants, local perfusion, and oxygen delivery, as well as enhances immune responses
- O3is a stimulator of the transmembrane flow of O2. The increase in O2 levels inside the cell secondary to O3therapy makes the mitochondrial respiratory chain more efficient.
- In red blood cells, O3-AHT may increase the activity of phosphofructokinase, increasing the rate of glycolysis. By enhancing the glycolytic rate, there is an increase in ATP and 2,3-diphosphoglycerate (2,3-DPG) in the cell. Subsequently, due to the Bohr effect, there is a rightward shift in the oxyhemoglobin dissociation curve allowing for the oxygen bound hemoglobin to be unloaded more readily to ischemic tissues.
Benefits of Ozone
- Speeds up the metabolic process
- Stimulates the immune system
- Increases circulation, oxygen and nutrient delivery within the body. Increase the amount of oxygen in the blood. Improves brain function and memory.
- Oxidizes toxins that have accumulated in the body such as pesticides, drug residues, acidic wastes and more. Toxins are then eliminated through the skin, lungs, kidneys and the colon.
- Purifies the blood and lymph. Balances hormones and enzyme production.
- Stimulates weight-loss and cellulite reduction
- Supports and enhances the healing process. Deactivates viruses, bacteria, eczema, fungi; etc. Ozone is effective in treating most allergies.
Ozone therapy and Holism
- According to father Sebastian kneipp (1821-1897), the 5 pillars of classic biological natural medicine are:
- Water therapy
- Nutritional therapy
- Therapy with medicinal plants
- Proper living
- Proper exercise
Thus the 5th pillar in this method: movement in fresh air as a “medical intervention”- a natural improvement in the O2 supply via physical activity, sport etc.
Ozone therapy as a form of oxygen therapy to stimulate mitochondria and give them a “breath of fresh air”.
6 Therapeutic effects of Ozone
- Improve Oxygen Metabolism
- Regulates Oxidative Stress
- Regulates of Prostaglandins
- Immunomodulator
- Germicidal Properties
- Metabolic Regulator
Methods of Ozone application
External application
- Ozonated oil
- Bagging
- Cupping
- Funneling
- Ozonated steam
Internal application
- Ozonated water
- Rectal insufflation
- Vaginal insufflation
- Ear insufflation
- Injections (PS/ PA/ SC/ IM Injections)
- Boto (breathing ozone through oil)
- Major and minor autohemotherapy.




Clinical applications of Ozone

Indications
- Psoriasis
- Diabetic foot
- Arthritis
- Painful conditions
- Cancer
- Sinusitis
- Asthma
- Eczema
Contraindications
- Glucose 6 phosphate dehydrogenase deficiency (individuals with a glucose-6-phosphate dehydrogenase deficiency may experience acute hemolysis on exposure to ozone)
- Hyperthyroidism (risk of ozone-induced lung toxicity is substantially increased in a hyperthyroid state and suggest that the susceptibility of the lung to damage from ozone exposure may be significantly influenced by individual thyroid hormone status)
- Cardiac instability
- Pregnancy
- blood-clotting disorders, including hemophilia, constant consumption of anticoagulants
- Conditions after hemorrhage and hemorrhagic tendency.
High patient acceptance of Ozone
- Effective
- Quick results
- Affordable
- No side effects
- Less time consuming
- Simple procedures
- Can be combined with alternative regular treatments